She was born on 10 December 1472[2] at Framlingham Castle in Suffolk, the only (surviving) child of John de Mowbray, 4th Duke of Norfolk and Lady Elizabeth Talbot.

Her maternal grandparents were John Talbot, 1st Earl of Shrewsbury and his second wife Lady Margaret Beauchamp.

On 15 January 1478, aged 6, she was married in St Stephen's Chapel, Westminster, to Richard of Shrewsbury, Duke of York, the 4-year-old younger son of King Edward IV and his Queen, Elizabeth Woodville.

[1] Anne died at Greenwich in London on 19 November 1481,[2][1] nearly two years before her husband disappeared into the Tower of London with his older brother, Edward V. Upon her death, her heirs normally would have been her cousins, William, Viscount Berkeley and John, Lord Howard, but by an act of Parliament in January 1483 the rights were given to her husband Richard, with reversion to his descendants, and, failing that, to the descendants of his father Edward IV.

Westminster Abbey is the presumed resting place of her husband, Richard Duke of York, and his brother Edward V, in the Henry VII Chapel.