Seguier married Francois du Prat, Baron of Vitteaux and of Thiern.
They had three children, Antoine du Prat who married Chrétienne de Sayve, Anne and Philippine, who were educated in the court of Henry III.
Seguier married again to Hugues de La Vergne, Chamberlain and Captain of the guard of the Duke of Anjou.
Anne became a lady of Queen Catherine de' Medici and in 1584 she married Honorat Prévost, Lord of Chastellier-Portaut.
[1][2][3][4][5][6] Seguier was considered a notable poet who wrote her work as a dialogue between Virtue, Honour, Pleasure, Fortune, and Death.