Anne of Gloucester, Countess of Stafford (30 April 1383 – 16 October 1438) was the eldest daughter and eventually sole heiress of Thomas of Woodstock, 1st Duke of Gloucester (the fifth surviving son and youngest child of King Edward III), by his wife Eleanor de Bohun, one of the two daughters and co-heiresses of Humphrey de Bohun, 7th Earl of Hereford, 6th Earl of Essex (1341–1373) of Pleshey Castle in Essex.

Her uncle, John of Gaunt (third son of King Edward III), ordered several payments to be made in regards to the event.

At the death of her brother Humphrey, 2nd Earl of Buckingham, in 1399, Anne was the co-heiress together with her two sisters Joan and Isabel, to his estates and titles.

[3][4] Anne became the sole heiress of the family's estate and titles in 1400, as one of her sisters, Joan, having died on 16 August 1400, and the other, Isabel, having become a nun.

They had three children together: In about 1405, Anne married William Bourchier, 1st Count of Eu (d. 1420), son of Sir William Bourchier and Eleanor of Louvain, by whom she had the following children: Anne died on 16 October 1438 and was buried in Llanthony Secunda Priory, Gloucester.