Annely Peebo gained a diploma for choral conducting and completed vocal studies in opera.

She studied at the University of Music and Dramatic Arts Vienna in Vienna, Austria, where she studied singing with Professor Gerhard Kahry and opera with Professor Curt Malm.

At the Vienna Festival in 2001, she sang the role of Meroe in the first performance of the expressionistic opera, Penthesilea by Othmar Schoeck (after Heinrich von Kleist).

Over the years, she has performed together with the likes of Plácido Domingo, José Carreras, Andrea Bocelli, Renato Bruson, and Stefania Bonfadelli.

Peebo speaks five languages, which allowed her to play a leading role in the French movie Les leçons des ténèbres as well as hosting the Eurovision Song Contest 2002 in Tallinn together with Marko Matvere.