The Annunciation Cathedral (Ukrainian: Свято-Благовіщенський кафедральний собор) is the main Orthodox church of Kharkiv, Ukraine.

The pentacupolar Neo-Byzantine structure with a distinctive 80-meter-tall bell tower was completed on 2 October 1888, from designs by a local architect, Mikhail Lovtsov.

[1] The church was frescoed in a style derived from St Vladimir's Cathedral in Kyiv.

Since 1946 the cathedral has been the seat of the Kharkiv and Bohodukhiv eparchy of the Ukrainian Orthodox Church (Moscow Patriarchate), while the Intercession Convent has served as the bishop's residence.

The Ecumenical Patriarch Athanasius III and several saintly bishops are buried in the cathedral.