Both the main painting, "Ansidei Madonna", and the predella "Saint John the Baptist Preaching", are located at the National Gallery in London.

This compares to the more natural poses and interaction found in Madonna, the Christ Child and infant John the Baptist in paintings of his Roman period.

[3] Each subject and the landscape of "Ansidei Madonna" evokes serenity and divinity: The three balls at Bishop Nicholas' feet may symbolize the holy trinity, or the three bags of gold he is said to have thrown into the window of a poor man's home for his daughters' welfare.

[3] Raphael's years in Florence exposed him to a plethora of artistic influences, first his teacher Perugino and then others, such as Donatello's sculptured marble, Masaccio's frescoes, Michelangelo's David, Leonardo da Vinci's paintings, and so much more which Raphael used to develop his fine-tuned sense of style, composition and execution as seen in the "Ansidei Madonna".

A master at the young age of twenty-three, Raphael brought new life to well-represented subjects, through careful, methodic performance.

[4] The Virgin Mary, Saint John and Bishop Nicholas are isolated from one another, without interchange, a style common in the Umbrian school, and particularly Perugino.

[10] The work was bought by young Lord Robert Spenser in 1764, for an undisclosed, but apparently large sum of money as a gift to his brother, the 4th Duke of Marlborough.

"[6][12] "Ansidei Madonna", considered "one of the most perfect pictures of the world",[3] of the Blenheim Collection was sold by George Spencer-Churchill, 8th Duke of Marlborough, under the Lord Cairn's Act for £75,000[13] or nearly unanimously cited at £70,000,[3][4] which was about $350,000,[14] to the London National Gallery in 1885.