The organization sees evolution as incompatible with the Bible and believes anything other than the young Earth view is a compromise on the principle of biblical inerrancy.

[14] The Answers in Genesis organization rejects key scientific facts and theories as established by archeology, cosmology, geology, paleontology, and evolutionary biology and argues that the universe, the Earth and life originated about 6,000 years ago.

[20] AiG has objected to the James Webb Space Telescope, saying "Sadly, many in this particular camp (sometimes ignorantly) have actually compromised Scripture by accepting the secular ideas being pushed by the JWST media at NASA (i.e., the big bang and evolution), thus rejecting the plain (biblical) reading of Genesis 1 and instead reinterpreting the days of creation to long ages.

This is an unbiblical way of thinking that essentially elevates man's fallible ideas as the ultimate standard (i.e., humanism) over the infallible Word of God".

A. Gill, a British writer and critic, described the museum as "battling science and reason since 2007", writing: "This place doesn't just take on evolution—it squares off with geology, anthropology, paleontology, history, chemistry, astronomy, zoology, biology, and good taste.

[31] In December 2016, for the holiday season, AiG lit the Ark with rainbow colors, aiming to "reclaim the symbol from the gay rights movement" and to remind viewers of the Noahic covenant.

[37][38][39] Each permanent employee of AiG including people who work at the museum must sign a statement of faith "in order to preserve the function and integrity of the ministry", indicating that they believe in young Earth creationism and the other teachings of Answers in Genesis.

[41][42] In 2007, The Kentucky Post reported that the Creation Museum employed between 10 and 20 security guards armed with .40 caliber Glock handguns and three certified law enforcement canines.

[56] In 1998, Answers in Genesis filmed an interview with Richard Dawkins, a prominent evolutionary biologist at Oxford University, resulting in a controversial video that AiG posted on its website.

The "suspiciously amateurish" interview included, according to Dawkins, "the kind of question only a creationist would ask in that way" (namely, to "give an example of a genetic mutation or an evolutionary process which can be seen to increase the information in the genome").

Realizing that he had been duped, Dawkins, at his admission, was angry at the thought and initially refused to answer the question but relented and continued the interview.

When I eventually saw the film a year later, I found that it had been edited to give the false impression that I was incapable of answering the question about information content.

[59] In the spring of 2009, Answers in Genesis posted a billboard in Texas with a young boy aiming a gun towards the viewer with the words, "If God doesn't matter to him, do you?"

The controversy stemmed from Ham's commentary on the position expressed by Peter Enns, of The BioLogos Foundation, who advocated a symbolic rather than a literal interpretation of the fall of Adam and Eve.

Writing on his blog, Ham accused Enns of espousing "outright liberal theology that totally undermines the authority of the Word of God", which led to his invitation being revoked.

[62] On May 31, 2007, Creation Ministries International filed a lawsuit in Supreme Court of Queensland against Ken Ham and Answers in Genesis seeking damages and accusing him of "unbiblical/unethical/unlawful behaviour" in his dealings with the Australian organization.

[64][65] An editorial analysis of the situation, including reference to estranged co-founder John Mackay's allegations in 1986 of necrophilia and witchcraft against Ken Ham's personal secretary is offered in an account in the Reports of the National Center for Science Education.

[66] In February 2009, the United States Court of Appeals for the Sixth Circuit ordered Australian-based Creation Ministries International into arbitration with Answers in Genesis over copyrights and control of affiliates in other countries.