In 1977 and 1978 Anthoupoli, under the guidance of coach Pavlos Grigoriadis reached the Larissa Cup final against experienced Achilleas Farsala winning the trophy on the first occasion.

[5] They maintained a powerful side in the late 1990s fighting for their promotion to Delta Ethniki and developed a strong relationship with one of the professional football clubs of the Larissa town, AEL which had as result in a few young players of Anthoupoli joining the latter, such as Stelios Tsiantoulis, Apostolos Tsianakas, Christos Gkikoudis and Vasilis Tahtintis.

In July 2022, local businessman Christos Priftis was elected to replace long-servingpresident Vassilis Adamopoulos with an aim to bring Anthoupoli back to the national divisions.

The football pitch is part of the Anthoupoli training complex, that includes basketball and tennis courts and it belongs to the municipality of Larissa.

[12] Anthoupoli used to share their ground with another local team Haravgi Larissa who they developed a great rivalry with in the 1980s and 1990s.