In 1893, he worked as demonstrator to the chair of pathological histology and bacteriology and as of 1894 as assistant of Anton Weichselbaum (1845–1920) at the pathological-anatomical institute at the University of Vienna.

[1] Anton Ghon travelled to Bombay in 1897 as a member of the Austrian delegation researching the bubonic plague.

For their findings on aetiology, anatomical pathology and epidemiology, he and his colleagues were nominated for the Nobel Prize in 1901.

In 1910 he became full professor of pathological anatomy at the German University in Prague succeeding Richard Kretz.

His body was transferred to his hometown Villach and laid to rest in the family tomb.