On 12 April 1612 he was appointed as maestro di capella to the Order of Saint Stephen at the Santo Stefano dei Cavalieri Church in Pisa in the service of the Grand Duke of Tuscany.

[1] His pupil, composer Giovanni Bettini, followed him to Pisa and was later appointed organist at the Santo Stefano dei Cavalieri in 1618, most likely by Brunelli.

Brunelli wrote and published several theoretical treatises, most notably the Regole utilissime per i scolari che desiderano imperare a cantare (Florence, 1606), one of the first published methods for voice.

[1] Emiliano Ramacci: Relazione dal XVII Convegno della Società Italiana di Musicologia (Pisa 2010).

Emiliano Ramacci: La famiglia di Antonio Brunelli a Bagnoregio in Musiche D'Ingegno, Pisa, Pacini, 1999.