The club was founded in 1930 by students of the Averofius Farm School of Larissa in a rural settlement of the city of Philipoupoli and its first name was "Dimitra".

[1] Twelve years later, in 1964, Apollon Larissa managed and played it for the first time in Greek second division, but did not win a league match at that level.

In the Greek Cup, Apollon Larissa biggest distinction was the 1997–98 season when they reached the quarter-finals, where they were eliminated by Iraklis.

In May 2011, Apollon Larissa was relegated to the regionalized fifth level of Greek football, ending a period of 31 years in the national third and fourth divisions.

Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.