Aquilegia bertolonii, common name Bertoloni columbine or Bertoloni's columbine,[3][4] is a perennial species of flowering plant in the family Ranunculaceae, native to Italy, southern France, and Slovenia.

In early summer each erect stem produces up to four spurred, blue-purple flowers.

The two species are estimated to have diverged from each other in the Pliocene around 1.23 million years ago, and form a sister clade to one containing the other European and some North and East Asian species of Aquilegia, from which they diverged approximately 2.5 million years ago.

[4] When first describing this species, Heinrich Wilhelm Schott noted that Bertoloni had classified a specimen as Aquilegia pyrenaica, but Schott considered this specimen to be too different for the identification to hold, instead describing it as a new species with Bertoloni's name.

[7] Aquilegia bertolonii is native to the northwestern Apennine Mountains in Italy,[2] also being found in southern France and three disjunct areas in the Slovenian Alps.