It holds and interprets artifacts from the Prehistoric, Archaic, Classical, Hellenistic and Roman periods, mostly from the city of Thessaloniki but also from the region of Macedonia in general.

The new wing hosts two exhibitions: The Gold of Macedon, with artefacts from the cemeteries of Sindos, Agia Paraskevi, Nea Filadelfia, Makrygialos, Derveni, Lete, Serres, and Evropos; and The Thessaloniki Area in Prehistory, with material from prehistoric settlements, dating from the Neolithic to the Early and Late Bronze Age.

These rooms display architectural members from an Ionic temple of the 6th century BC, sculptures of all periods from Macedonia, exhibits from the excavations in the palace complex built by Galerius in the Thessaloniki city centre, a reconstruction of the façade of the Macedonian tomb in Agia Paraskevi, with genuine architectural members, and finds (mainly gold artefacts) of the Archaic and Classical periods from the Sindos cemetery.

It presents the first excavations, which were carried out during the First World War by British and French troops, and finds from the most important prehistoric settlements in the area (Thermi, Vassilika, Stavroupoli, Oraiokastro, Assiros, Toumba, and Kastana) divided into three chronological groups (Neolithic, Early and Late Bronze Age).

[2] On 26 February 2010, Greek authorities arrested two men found in illegal possession of various antiquities, including a bronze statue of Alexander, which is possibly a work of Lysippos.