Founded in 1945, Ardley spent the majority of their early years in local competitions, primarily the Lord Jersey League,[2] winning the Lord Jersey Cup in 1970 and Division One and the Charity Cup in 1984–85.

The 1990–91 season saw the club win the Premier Division and the Archie Goddard Cup.

[3] In 2013–14 they won the Oxfordshire Senior Cup for the first time, beating Kidlington 4–0 in the final.

[5] The 2016–17 season saw Ardley win the Supplementary Cup, beating Tuffley Rovers 2–0 in the final.

At the end of the 2020–21 season the club were transferred to the Premier Division of the Spartan South Midlands League.