[2] In southern Calhoun County, Highway 160 begins at US Route 278 (US 278) and runs east to US 425 in Fountain Hill.

[3][4] A fourth segment begins at US 82 and runs 22.73 miles (36.58 km) east to US 65 at Chicot Junction.

The route runs underneath Interstate 49 before it crosses the Red River to enter Lafayette County.

The route enters Columbia County near Taylor where it forms a 0.13 miles (0.21 km) officially designated exception over US 371[8] before continuing northeast to Macedonia where it terminates at Highway 19.

[10] Arkansas Highway 160 travels south until meeting AR 172, after which it begins to arrow east to join with US 63/AR 15.