The route of 5.00 miles (8.05 km) runs from US 61 in Osceola north through the city to terminate at Highway 158.

The route intersects Highway 239 before it forms a concurrency with US 61 for 0.7 miles (1.1 km).

Highway 325 passes the Hale Avenue Historic District, City Hall, and the Florida Brothers Building all listed on the National Register of Historic Places.

The route also passes within a block of the Mississippi County Courthouse.

Another overlap begins westbound with AR 119 for another 0.7 miles (1.1 km).