In 2000, he received his clinical residency training from the same university with a specialization in forensic medicine.

Ashotyan joined the Republican Party of Armenia (RPA) in 2002 and from 2003 to 2005 served as the chairman of its youth wing.

Ashotyan was first elected to the National Assembly of Armenia in 2005 by the proportional system of voting, and was a member of the RPA's parliamentary faction.

[2] In September 2020, when the Second Karabakh War broke out, he was called into the Armenian armed forces and served in the medical corps for the duration of the conflict.

Since the end of the conflict, he has been instrumental in leading anti-government protests that seek to oust Prime Minister Nikol Pashinyan.