Arthur Jeffrey Dempster (August 14, 1886 – March 11, 1950) was a Canadian-American physicist best known for his work in mass spectrometry and his discovery in 1935 of the uranium isotope 235U.

After spending a semester each at Gottingen and Munich, Germany, and two years at the University of Wurzburg under Wilhelm Wien he left at the outset of World War I for the United States; there he completed his Ph.D. in physics, graduating summa cum laude, at the University of Chicago originally begun in Germany under Wien.

Dempster used a mass spectrometer of his design, in 1935 to find uranium-235, an isotope of uranium which is lighter than uranium-238 and allowed for the production of atomic weaponry, and later energy.

[2][3] From 1943 to 1946, Dempster was chief physicist of the University of Chicago's Metallurgical Laboratory or "Met Lab" which integrally related to the Manhattan Project and was founded to study the materials necessary for the manufacture of atomic bombs.

Dempster's research over his career centered on the mass spectrometer and its applications, leading in 1935 to his discovery of the uranium isotope 235U.