Assiniboia is a provincial electoral division in the Canadian province of Manitoba.

It was first created for the 1879 provincial election, was eliminated in 1888, and was re-established in 1903.

It is located in the westernmost tip of the City of Winnipeg.

Between 1920 and 1949, it was a hotly contested riding between the Conservatives and candidates of the Independent Labour Party and Manitoba Co-operative Commonwealth Federation (CCF).

The New Democratic Party of Manitoba's (NDP) victory in 1999 was completely unexpected, and occurred by a margin of only three votes.