Operated from the outset by the Great Western Railway it stayed part of that company after the Grouping of 1923.

A single track ran through the station then on under a bridge carrying the A40 main road between Oxford and London.

There were reports in 1997 that the Chinnor and Princes Risborough Railway (CPRR) wished to extend its operations to Aston Rowant.

[5] A joint venture between the CPRR and Chiltern Railways was also proposed whereby the national rail operator would construct a new station at Aston Rowant to allow frequent weekday commuter services along the Icknield Line to connect with main line traffic through to London Marylebone, leaving the CPPR to run heritage services at other times.

[6] The scheme, which would cost around £3m, would seek to take advantage of Aston Rowant's location near junction 6 of the busy M40 motorway.