The men's 100 metres at the 2000 Summer Olympics as part of the athletics program were held at the Stadium Australia from 22 to 23 September.

Obadele Thompson won the first-ever medal in the men's 100 metres for Barbados with bronze.

Two-time silver medalist Frankie Fredericks of Namibia was injured and unable to compete.

[2] Albania, American Samoa, Brunei, Croatia, Georgia, Guam, Palau, and Saint Lucia appeared in the event for the first time.

For the men's 100 metres, each National Olympic Committee was permitted to enter up to three athletes that had run the race in 10.27 seconds or faster during the qualification period.

The event retained the same basic four round format introduced in 1920: heats, quarterfinals, semifinals, and a final.