The women's 400 metres T54 event at the 2024 Summer Paralympics in Paris, took place on 5 September 2024.

The T54 category is for wheelchair athletes with no leg function, but near full arm function and reasonable to normal trunk function.

Typically this may be caused by a lower spinal cord injury or spinal cord birth defect.

Prior to the competition, the existing records were as follows:[1] First 3 in each heat (Q) and the next 2 fastest (q) advance to the Final.