His brother Alfred was for many years the principal horn player in the Queen's Hall Orchestra under Sir Henry Wood.
He was appointed principal horn of the New Symphony Orchestra that same year, and in 1912 he went on the LSO's tour of the United States under Arthur Nikisch.
In 1923 he succeeded his teacher, Adolf Borsdorf as professor of horn at the Royal Academy of Music, where his son Dennis was among his students.
Dame Ethel Smyth wrote her Concerto for Violin, Horn and Orchestra with Aubrey Brain in mind.
He had a marked preference for French instruments, and played a hand horn made by Labbaye in c. 1865, to which English-made valves had been added.