Augusto Ruschi Biological Reserve (Portuguese: Reserva Biológica Augusto Ruschi) is a Federal biological reserve in the state of Espírito Santo, Brazil.

It was created on 20 September 1982 and is administered by the Chico Mendes Institute for Biodiversity Conservation.

Streams rising in the reserve flow into the Doce, Timbuí and Piraqueaçú rivers.

[3] Protected birds in the reserve include the red-browed amazon (Amazona rhodocorytha), white-necked hawk (Leucopternis lacernulatus), Atlantic black-breasted woodpecker (Celeus torquatus tinnunculus), rufous-brown solitaire (Cichlopsis leucogenys), purple-winged ground dove (Claravis godefrida), black-bellied thorntail (Discosura langsdorffi), Salvadori's antwren (Myrmotherula minor) and brown-backed parrotlet (Touit melanonotus).

Protected mammals include the maned sloth (Bradypus torquatus), Atlantic titi (Callicebus personatus), northern muriqui (Brachyteles hypoxanthus), buffy-headed marmoset (Callithrix flaviceps), ocelot (Leopardus pardalis) and cougar (Puma concolor capricornensis).