Australia competed at the 2013 World Aquatics Championships in Barcelona, Spain from 19 July to 4 August 2013.

* – Indicates the athlete competed in preliminaries but not the final relay.

[1] Australian swimmers achieved qualifying standards in the following events (up to a maximum of 2 swimmers in each event at the A-standard entry time, and 1 at the B-standard):[1][2][3] Cameron McEvoy tied for the eighth and final spot in the final of the 200 m freestyle with Sebastiaan Verschuren from the Netherlands.

However, Verschuren withdrew from the swimoff to focus on the 100 m freestyle event which allowed McEvoy to advance to the final.

[4] Reserves Australia at other World Championships in 2013