Ayano Nagai (born 永井彩乃 14 October 1997) is a Japanese rugby union player.

She plays for Japan at an international level and competed in the 2021 Rugby World Cup.

[2] In 2022, she featured in the Sakura's victory against Ireland at Prince Chichibu Memorial Stadium.

[3] She was later selected in Japan's side for the delayed 2021 Rugby World Cup that was held in New Zealand.

[8][9] Her try in the 16th minute helped her side defeat Kazakhstan and secure a spot at the 2025 Rugby World Cup and the 2024 WXV 2 competition.