They are currently members of Southern League Division One Central and play at Chesham United's Meadow ground.

With the league becoming a single division in 1910, the club remained in it until World War I, finishing as runners-up again in 1913–14.

[4] Further league reorganisation at the end of the season saw the creation of a Premier Division, in which Aylesbury were placed.

Several clubs left the league at the end of the season, resulting in it being reduced to a single division.

[5] In 1951 Aylesbury were founder members of the Delphian League, and the 1951–52 season saw them reach the first round of the FA Cup for the first time.

[3] The following season saw them reach the first round of the FA Cup again, this time losing 5–2 to Slough Town in a replay after a 2–2 draw.

In 1987–88 they won the Premier Division, earning promotion to the Football Conference; they also reached the first round of the FA Cup for the third consecutive season, this time losing 1–0 at Bristol City.

Another first round defeat took place in 1992–93 when they were beaten 8–0 at West Bromwich Albion, but the 1994–95 season saw their best performance to date, reaching the third round after defeating fellow non-League clubs Newport (IOW) and Kingstonian before losing 4–0 at QPR.

The run included the "duck walk" celebration by Cliff Hercules, the club's record appearance holder and goalscorer,[9] which was featured on the BBC's They Think It's All Over programme.