In 1960, Ayr winger Jim Black was chosen for the Inter-City match, a rare honour for a player not with an Unofficial Championship club.

Winger David Ashton won his B cap against France and in the following year John Brown was in the B side at full back in the same fixture.

Earlier in that decade, loose-head prop Jock Craig had sat on the bench for the Scotland B side, and been chosen for the International Trial.

In 1980 Stephen Munro won the first of his ten full caps, against Ireland, the last coming in the Welsh match of the 1984 Grand Slam season.

Other Ayr players who gained international caps at other clubs were Gordon Strachan, Quintin Dunlop, Derek Stark, Derrick Lee.

Mark Bennett, now with Edinburgh, whose career began at nearby Cumnock, joined Ayr, aged 17 and played for the club prior to moving-on the Glasgow Warriors and Scotland.

Ross Curle, Robbie Fergusson (now co-captain of the Scotland Sevens squad), Stuart Fenwick, Danny McCluskey, Grant Anderson, Kerr Gossman, Stafford McDowall, Robbie Smith, Matt Davidson, Marshall Sykes, Euan McLaren and Paddy Dewhirst are just a few of the current and recent Ayr squad who have represented Scotland at age grade level.

Scott Sutherland, Craig Gossman, Dean Kelbrick, Andrew Dunlop, Nick Cox, Callum Templeton, Robin McAlpine, Steven Longwell, Blair Macpherson, Pete McCallum, David Armstrong, Damien Kelly have all played for the Scotland Club XV.

On 23 February 2013, with one game remaining, a 39–13 victory over Currie, the reverse fixture being the only defeat for Ayr in the league, coupled with the only challengers Gala Rugby losing, meant the Scottish Premiership returned to Millbrae.

In the Cup Final, at Murrayfield, long-serving Kiwi stand-off Frazier Climo kicked the match-winning penalty, from the touchline, in time added on for injuries.

In the new season, most of the first team squad moved across to Ayrshire Bulls, to play in the FOSROC Super6, with the 'Club XV' being placed, along with the other five Super6 "Club XVs" in National League 1.

In the first year of the competition, Ayr finished 4th in group D having lost to Doncaster (A), Cardiff (A), Pontypridd (H) and beating Rotherham (H) and Birmingham and Solihull (H).

In the second year of the competition, Ayr were pooled with the same teams in group D again (apart from the replacement of Cardiff by Llandovery) with the location of the fixtures being reversed.

Ayr finished second in the group, losing to Pontypridd (A), drawing with Rotherham (A) and beating Birmingham and Solihull (A), Llandovery (H) and Doncaster (H).