In 1923, the board gave one of the members, Svend Zachariasen, permission to erect an ice cream parlour for an annual payment of DKK 110.

[1] In 1928, Marienlyst had a pitch and clubhouse on the opposite side of Skibhusvej, by Sandhusvej, which became the club's home ground for almost half a century.

Marienlyst was enrolled in Fyns Boldspil-Union (DBU Funen) in 1923 and was placed in the FBU B-række division, the third regional tier of the local association.

However, due to the favourable geographical location in an area with many families and youths, the club quickly became one of Funen's most prominent.

The current home ground of the club, Marienlystcentret, was inaugurated during this decade, as was the fanclub Boldklubben Marienlysts Venner.

The facilities on Sandhusvej had become too cramped, and in the early 1970s club management succeeded to create the possibility relocating to a large municipal plot between Windelsvej and Hedvigslundskoven.

The club's record for first team appearances is held by Lars Petersen with 432 (between 1972–1995), followed by Allan Hoffmann Nielsen (1973–1991) with 409 and Preben Hansen (1970–1988) with 320.