The Cathedral of Barbastro (Spanish: Catedral de Santa María de la Asunción) is a Roman Catholic cathedral in the town of Barbastro in the province of Huesca, Aragon, Spain.

A church was first erected on May 5, 1101, at the site of a mosque by Saint Poncio, bishop of the diocese.

The central and main altarpiece has scenes dedicated to the Assumption of the Virgin and Passion of Christ with an alabaster base sculpted in a Plateresque in the style of Damián Forment.

The chapel of San Jose has an altarpiece dedicated to the Abbot St Victorián, and is attributed to Bartolomé Bermejo or his studio.

One of the southern chapels, the Chapel of Miracles, has a venerated wood icon entitled the Santo Cristo de los Milagros, (Christ of the Miracles), carved by Enrique Monjó in 1939 at the end of the Spanish Civil War.