Barnsley Women's Football Club is an English Women's Football Club based in Barnsley, South Yorkshire, England.

The club currently plays in the FA Women's National League Division One North, the fourth tier of the English women's football league system.

In 2019/20 Barnsley Women had their best ever FA Cup run when they reached the fourth round of the Women's FA Cup, losing 5-0 away to professional side Tottenham Hotspur Women in an exciting match on Sunday 26 January 2020.

This was the largest attendance for a Women's FA Cup match, other than for Finals played at Wembley.

The club's junior teams play in the Sheffield & Hallamshire Women & Girls Leagues.