The club currently play in the fifth tier of English women's football.

Affiliated with the men's club, the new ladies team was announced on 18 June 2020,[1] and were placed in the Sheffield & Hallamshire Division One for their inaugural season.

[2] The season was abandoned due to the COVID-19 pandemic, but the club were granted promotion to the North East Regional Division One South.

[6] As part of its ongoing development initiatives, Barnsley FC is committed to improving player pathways and technical development, working with Reds in the Community and Barnsley Ladies.

[7] Note: Flags indicate national team as defined under FIFA eligibility rules; some limited exceptions apply.