In 1545 he published a single edition of a text by Sophocles, though he bequeathed manuscripts of the Iliad and Odyssey and works by Aristophanes, Pindar, Strabo, Lucian, Apollonius and other ancient Greek authors to the library at Sant'Agostino (the future biblioteca Angelica).
A document dating to 1560 shows he belonged to the 'Confraternita degli orfani e delle orfane', a charitable institution looking after male and female orphans which was supported by cardinal Giovanni Morone, with a fellow scholar of Marliani's.
A translation into Italian by Ercole Barbarasa was published in 1548 and until the 18th century Marliani's work was being reprinted in Italy and other countries.
The work accurately located the Roman Forum, but that aspect was obscured by a fierce debate with Pirro Ligorio, who in Christian Hülsen's words was a "famous architect ... but as an antiquarian [only] an ambitious dilettante".
This forced Marliani to add an addendum to a 1553 reprint of 'Topographia', in which a reborn Romulus claims a different site for the Roman Forum, but the author replies to him "Romulus, having just crossed the Lethe and thus forgotten all the places in the city, you swear the same nonsense as Strepsiades.“[3] Ligorio emphatically supported his theory using invented inscriptions and monuments and so his opinion prevailed until early 19th century excavations proved Marliani to have been correct.