To celebrate Batman's 80th anniversary, DC Comics and Warner Bros. lit the Bat-Signal in thirteen cities on September 21, 2019, starting in Melbourne and ending in Los Angeles.

After Batman departs, Gordon looks out at the city and considers the exceptional view from his current position, hinting at the future creation of the Signal.

The Bat-Signal seldom appeared in the 1960s TV series, Commissioner Gordon generally contacting Batman using a dedicated phone line (the Batphone).

However, the Bat-Signal was occasionally used (for instance, in the episode "The Sandman Cometh" when Bruce Wayne and Dick Grayson are away on a camping trip), whenever Batman needed to be summoned from the field.

The animated background for the closing credits of the TV series depicted the Bat-signal in the night sky over Gotham City.

[citation needed] In the finale of the fourth season, "A Dark Knight: No Man's Land", James Gordon has Lucius Fox activate the Floodlight on top of the G.C.P.D building.

During No Man's Land in the fifth season, Gordon continues using the signal as a symbol of hope for the good people left in Gotham and later meets with Bruce at the Floodlight in "Year Zero".

In the series finale "The Beginning...", Commissioner Gordon and Harvey Bullock re-ignite the searchlight to celebrate Bruce Wayne's return to Gotham after ten years.

However, having noticed the searchlight that illuminates the sky, the Dark Knight then appears on a building from across the street, watching Gordon, Bullock, and Alfred.

In the CW series The Flash, a 'Flash signal' is created by Cisco, who claimed to have gotten the idea from "some comic book", which implies Batman does not exist in on Arrowverse Earth One.

In the pilot episode of Batwoman, Gotham City Mayor Michael Akins was planning to turn off the Bat-Signal forever due to Batman's disappearance.

The Bat-Signal appears in the season finale of Titans, titled "Dick Grayson", in a dream world created by Trigon.

The signal is used when Commissioner Gordon needs Batman's help when the Red Triangle Circus Gang attack Max Shreck during Christmas and appears again at the end of the film as a surviving Catwoman looks on.

A music video for "Kiss from a Rose", also from Batman Forever, features singer Seal performing the song while standing near the Bat-Signal.

At the end of the film, the Bat-signal appears, as a searchlight that projects the shape of a bat, installed atop police headquarters as a means to contact Batman.

The Bat-Signal is a major plot point in the 2022 film The Batman, directed by Matt Reeves who co-wrote the screenplay with Peter Craig.

Batman in his opening monologue states that the signal also creates another purpose of spreading fear among Gotham's criminal element, as a warning.

After talking to Detective Yin, Batman is standing in front of the lighthouse light when the Bat Signal appears in the sky.

In the DC Super Hero Girls animated short "#BatCatcher", Batgirl mistakenly believes she is summoned by the Bat-Signal when in reality the shadow is cast from a real bat inside her bedroom.