Twaalfhoven received a scholarship at Fordham University in New York, and worked as a dishwasher, waiter, chauffeur and fruit-picker during the four years of his education.
Twaalfhoven used the then unknown method of Artificial Insemination, and in five years raised cows that increased milk production by 100%.
Twaalfhoven's sold the farm in 1958 and moved his family to Hilversum, The Netherlands, where he started a business, setting up the first laundromats in northwestern Europe.
[citation needed] In 2001 he received the Annual Alumni Award from Harvard Business School for his activities stimulating entrepreneurship in Eastern and Western Europe.
[3] Through his company Indivers B.V.,[4][5] Twaalfhoven started 54 businesses in the countries Italy, France, Germany, Russia, Ukraine, Holland, Belgium, England, U.S.A., Singapore and China.