Bethulie is a small sheep and cattle farming town in the Free State province of South Africa.

In 1828 a mission station was established by the London Missionary Society for the local people, the San Bushman.

It was originally known as Groot Moordenaarspoort (Murderer's Pass) after a vicious clash between the Sotho and Griqua tribes.

It now houses a historical museum displaying items of the past and information on the life and trial of Jean Pellissier and Chief Lephoi and his people.

Bethulie's most famous son was Patrick Mynhardt, whose one-man shows featuring Herman Charles Bosman's character Oom Schalk Lourens were popular in South Africa.