Southwest of the village centre was the wali of Sheikh Abu Talal, possibly built on the ruins of a Crusader church.

[5][6] Northeast of the village is Horvat Diab, an archaeological site containing the remains of a Jewish farmhouse or estate of the Second Temple period, along with rock-cut tombs in the kokhim style, and the ruins of a public structure that may have been a synagogue.

[13] Albert Socin, citing an official Ottoman village list compiled around 1870, noted that Biddu had 70 houses and a population of 247, although this count included only men.

Biddu was subjected to a short bombardment from a Davidka after which Palmach sappers entered the village and demolished its houses,[27][28] and effectively stripped it of its inhabitants.

Before withdrawing from both Biddu and Beit Surik, under Moshe Dayan's direction, a special unit contaminated the villages' wells with a biological warfare agent consisting of typhus and diphtheria bacteria.

The purpose of such poisoning was to make Palestinian villages that had conquered but not yet occupied uninhabitable to residents seeking to return to their homes.

[36] Biddu has become a focal point for non-violent resistance to the process of incorporating Palestinian lands into Israeli settlements.

[37] Diaa' A-Din 'Abd al-Karim Ibrahim Abu 'Eid was shot dead by gunfire during an anti-barrier demonstration on 18 April 2004.

[38] Muhammad Fadel Hashem Rian and Zakaria Mahmoud 'Eid Salem were shot dead during anti-barrier demonstrations on 26 February 2004 at Beit Ijaz (a satellite village of Biddu).