Biel/Bienne District is a constitutional district (Amtsbezirk) in the canton of Bern in Switzerland with its seat Biel/Bienne.

It is bi-lingual (41% French and 59% German) and includes two municipalities in an area of 25 km2: From 1 January 2010, the district lost its administrative power while being replaced by the administrative district of Biel/Bienne.

Since 2010, it remains a fully recognised district under the law and the Constitution (Art.3 al.2) of the canton of Bern.