It is based on the 2006 revival in Finland, Australia, United Kingdom and United States; developed by Creat Studio for the PlayStation 2, and British company Full Fat for the Nintendo DS; published by The Game Factory; and first released in Europe in 2006 and 2007.

On Mars, a battle wages on between the race of mice, known as the Martian Freedom Fighters, and the invading Catatonian Empire.

After their arrival, they land in Chicago and meet up with Charley Davidson, who runs and owns the Last Chance Garage, which becomes the Biker Mice's base of operations.

Not long afterwards do they realize the Catatonians, led by General Hairball and Cataclysm, have come to Earth in search of Stoker, so that they can ensure their claim of the Regenerator.

Throughout the course of the game, the player takes on the role of either Throttle, Vinnie and Modo, and maneuvers in and out of boss battles and other such levels.