[2] Distribution of Biomphalaria pfeifferi include: The type locality is in Umgeni Valley, KwaZulu-Natal, South Africa[1] (in the times of the description it was the British Colony of Natal).

[7] Biomphalaria pfeifferi can survive up to 16 hours in anaerobic water using lactic acid fermentation.

[8] In Kenya, B. pfeifferi is positively associated with the common blue water-lily Nymphaea caerulea (the two species occur together).

[3] Parasites of Biomphalaria pfeifferi include the following 11 species found in Tanzania:[9] In Kenya, releasing the edible American crayfish Procambarus clarkii as an introduced species has helped eliminate the mollusc, which it feeds on, as well as provided a new source of food and income,[3][11] but may also be impacting the environment by reducing the amount of native aquatic plants.

[13] Ethanolic extract of the fruits of Dalbergia sissoo (family Leguminosae) exhibited molluscicidal effect against eggs of Biomphalaria pfeifferi.