The cemetery was built in 1884 and was added to the National Register of Historic Places on November 11, 2010.

President C. Jeanne Táborský and her organization have worked to maintain and repair the cemetery grounds and turn a small building at the cemetery into a museum and cultural center called the "Bohemians of Baltimore Museum".

Since the neighborhood of Little Bohemia is long dispersed, the Bohemian National Cemetery is one of the few remnants of the Czech culture remaining in Baltimore, so the Č.S.P.S.

[5] John Waters mentions both the cemetery and the surrounding neighborhood in his book Role Models: Armistead Gardens, a neighborhood originally built as public housing for the influx of people coming to work in factories during World War II.

There is an amazing graveyard nearby where the star of my early movie Eat Your Makeup, Maelcum Soul, is buried.