Botanical Garden of Vilnius University (Lithuanian: Vilniaus universiteto botanikos sodas) is a botanical garden situated in Vilnius, Lithuania.

The garden was established by professor Jean-Emmanuel Gilibert of Vilnius University in 1781.

In 1919, the Botanical Garden of the Polish Stefan Batory University was started in a new location, in Vingis (known as Zakret at that time).

There is also a department of the garden in Vingis Park (address: M. K. Čiurlionio 110, LT-03100 Vilnius).

[2] The botanical garden carries out research in the areas of biotechnology, horticulture, molecular genetics, conservation, ethnobotany, systematics and taxonomy.