Bothrocara brunneum, the twoline eelpout, is a bathydemersal species of fish of the family Zoarcidae.

[1] The species is the only fish of the eelpouts to have two lateral lines and short blunt-ended gill rakers.

[2] The species has a wide range across the northeastern Pacific Ocean, between the Bering Sea and the shores of San Francisco,[3] and from Attu Island to the Baja California Peninsula.

The twoline eelpout has been found to have two lateral lines, the only one of its family, and also has a small blunt-ended gill raker.

[6] It has been hypothesized that, judging by an analysis of stomach content, the species feeds primarily on bottom-dwelling creatures, habitually eating anything it comes across that has food value and that is small enough to ingest.