In 2008 was followed by a Nintendo DS version on January 8, an Xbox Live Arcade release on March 12, and a PlayStation 3 launch on November 27.

The game is structured like Big Brain Academy in that puzzles are divided into four separate categories: Logic, Math, Visual, and Focus; the Xbox Live Arcade version, PlayStation 3, PSP, Wii, Nintendo DS, and PC add a fifth category, Memory.

In the Trout Route test, the player must follow a path based on the progressive numerical relationship given (i.e. +2, -3, etc.).

The game features two modes: The XBLA, PlayStation 3, and Nintendo DS versions also feature additional modes: The Nintendo DS version supports up to three player multiplayer via the Nintendo Wi-Fi Connection, while the Xbox Live Arcade, PlayStation Network, and OnLive versions features up to four player offline and online Xbox Live multiplayer, as well as online leaderboards.

[25] Another sequel, Brain Challenge 3: Think Again!, was released by Gameloft for mobile, iPod Touch, iPhone in September 2009.