The trains operate ScotRail services in the Ayrshire and Inverclyde regions of Scotland and had originally been intended for the cancelled Glasgow Airport Rail Link.

[9] The reliability issues and extended commissioning period resulted in an initially reduced service on parts of the ScotRail network, including the newly re-opened Airdrie-Bathgate line.

As of 2025[update], the Class 380 operates trains between Glasgow Central and Ayr, Largs, Ardrossan, Gourock, Wemyss Bay, Neilston, Newton, Barrhead and Cathcart Circle.

In addition, they also operated trains between Edinburgh and Glasgow Queen Street via Falkirk Grahamston, North Berwick and Dunbar for a time whilst awaiting the Class 385 introduction to service.

[12] Following the December 2014 timetable change, with the electrification of the Whifflet Line, services to Lanark were re-routed into Glasgow Central High Level.