Broth microdilution

Broth microdilution is a method used to test the susceptibility of microorganisms to antibiotics.

[2] During testing, multiple microtiter plates are filled with a certain broth, according to the needs of target bacteria.

If the broth became cloudy or a layer of cells formed at the bottom, then bacterial growth has occurred.

[1] The broth microdilution method can be used to test the susceptibility of microorganisms to multiple antibiotics at once.

The accuracy of its results are comparable to agar dilution, the gold standard of susceptibility testing.