Its history has been interwoven with that of Mohyla Academy which now occupies the remaining monastery buildings.

Patriarch Theophanes III of Jerusalem had it reorganized as a local brotherhood school, hence the name.

[1] Its benefactors included Petro Sahaidachny (whose tomb was on the grounds[2]), Petro Mohyla (who raised its status to that of collegium), and Ivan Mazepa (who asked Osip Startsev to design the five-domed katholikon in a style known as Mazepa Baroque).

On April 7, 1640, the abbot of the monastery, Leontiy, wrote a "petition" to the Tsar of Muscovy to restore the Church of the Three Saints in Kyiv.

The remaining buildings have been either reduced to ruins[2] or rebuilt with significant alterations (as was a refectory church, dating from the 17th century).