Bruno Hassenstein (23 November 1839 – 27 August 1902) was a German cartographer born in Ruhla.
In 1866 he relocated to Berlin, where one of his duties was cartographical work for Karl Klaus von der Decken's Reisen in Ostafrika.
In 1868 Hassenstein returned to Gotha, where he created maps for Heinrich Theodor Menke's edition of Spruner's atlas of medieval history (Handatlas für die Geschichte des Mittelalters und der neuern Zeit).
In 1878 he became editor of the cartography section of the Mittellungen, and in 1885 performed mapmaking work for the Atlas von Japan, a highly regarded work containing charts that were drawn to 1:100000 scale.
During his career Hassenstein edited and published maps based on the observations of explorers and travelers (Wilhelm Junker, Emin Pasha, Hans Meyer, Ernst Marno, Friedrich Gerhard Rohlfs, Oskar Baumann, Friedrich Bohndorff and Sven Hedin).