He made his first-team debut on 13 February 2010, starting in a 1–0 away loss against Gimnasia La Plata for the Argentine Primera División championship, and aged only 16.

[4] In the 2012–13 season, after the departures of Giovanni Moreno and Lucas Nahuel Castro, Zuculini was an ever-present figure for the Avellaneda side, appearing in 32 matches and scoring five goals.

[7] Zuculini made his competitive debut two days later in the 2014 FA Community Shield, replacing Yaya Touré after 60 minutes of an eventual 3–0 defeat to Arsenal at Wembley Stadium.

[10] He made his debut in the competition on 29 August 2014, coming on at half time for André Gomes in a 3–0 home win against Málaga CF.

[17] On 23 February 2016, Argentine midfielder Zuculini suffered a serious fracture injury in the metatarsal and had to return to England in order to undergo surgery.