The station was acquired by the Detroit and Milwaukee Railway, and also served the Michigan Southern & Northern Indiana Railroad.

The station was destroyed by fire on the evening of April 26, 1866, when someone with a lantern went to inspect a leaking barrel of naphtha being loaded onto a freight car, setting off a chain reaction which also destroyed the ferry boat Windsor moored along the river, killing 17 passengers on the ferry and one person on a passenger train.

The Grand Trunk Western Railroad Detroit-Port Huron trains begin using the Brush Street Station in 1928.

[5] Continuing at the station, past the May 1, 1971 shift of American passenger trains to Amtrak, was the Canadian National Railway's/Grand Trunk's Tempo service from Detroit to Toronto via Windsor and London.

The site is now the surface parking lot and the rail line to it repurposed as the Dequindre Cut greenway.